SMAD7 Antibody (aa49-317)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P |
---|---|
Primary Accession | O15105 |
Other Accession | 4092 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 46426 Da |
Dilution | IHC-P (10 µg/ml), |
Gene ID | 4092 |
---|---|
Other Names | SMAD7, CRCS3, HSMAD7, MAD homolog 7, SMAD 7, MADH7, MADH8, Mothers against DPP homolog 7, Mothers against DPP homolog 8, MAD homolog 8, SMAD family member 7 |
Target/Specificity | Human SMAD7 |
Reconstitution & Storage | 0.1 M Tris-glycine, pH 7, 10% Glycerol, 0.01% Thimerosal. Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
Precautions | SMAD7 Antibody (aa49-317) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SMAD7 |
---|---|
Synonyms | MADH7, MADH8 |
Function | Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access (PubMed:21791611). Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
Cellular Location | Nucleus. Cytoplasm. Note=Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236) |
Tissue Location | Ubiquitous with higher expression in the lung and vascular endothelium |
Volume | 50 µl |
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Background
Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A- PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity).
References
Hayashi H.,et al.Cell 89:1165-1173(1997).
Topper J.N.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:9314-9319(1997).
Nakao A.,et al.Nature 389:631-635(1997).
Hagiwara K.,et al.Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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