|Application ||WB, IHC-P, E|
|Calculated MW||40971 Da|
|Dilution||ELISA (1:20000), IHC-P (5 µg/ml), WB (1:500 - 1:1000),|
|Other Names||DHPS, Deoxyhypusine synthase, DS, DHS, MIG13, Migration-inducing gene 13|
|Target/Specificity||DHPS Antibody detects endogenous levels of total DHPS protein.|
|Reconstitution & Storage||PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol. Store at -20°C for up to one year.|
|Precautions||DHPS Antibody (aa51-100) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.|
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Provided below are standard protocols that you may find useful for product applications.
Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.
Joe Y.A.,et al.J. Biol. Chem. 270:22386-22392(1995).
Bevec D.,et al.FEBS Lett. 378:195-198(1996).
Yan Y.P.,et al.Biochem. J. 315:429-434(1996).
Mantuano E.,et al.Gene 215:153-157(1998).
Yu W.,et al.Genome Res. 7:353-358(1997).
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