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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   COMMD1 Antibody (clone 2E2)   

COMMD1 Antibody (clone 2E2)

Mouse Monoclonal Antibody

     
  • IHC - COMMD1 Antibody (clone 2E2) ALS17048
    Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
  • IHC - COMMD1 Antibody (clone 2E2) ALS17048
    Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
  • WB - COMMD1 Antibody (clone 2E2) ALS17048
    HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COMMD1...
  • WB - COMMD1 Antibody (clone 2E2) ALS17048
    Western blot of extracts (35 ug) from 9 different cell lines by using g anti-COMMD1 monoclonal...
  • FC - COMMD1 Antibody (clone 2E2) ALS17048
    Flow cytometry of HeLa cells, using anti-COMMD1 antibody (Red), compared to a nonspecific...
  • FC - COMMD1 Antibody (clone 2E2) ALS17048
    HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid...
  • FC - COMMD1 Antibody (clone 2E2) ALS17048
    Flow cytometry of Jurkat cells, using anti-COMMD1 antibody (Red), compared to a nonspecific...
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, Flo
Primary Accession Q8N668
Other Accession 150684
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names 2E2
Calculated MW 21178 Da
Dilution Flo (1:100), IHC-P (10 µg/ml), WB (1:2000),
Additional Information
Gene ID 150684
Other Names COMMD1, C2orf5, Protein Murr1, Copper metabolism gene MURR1, MURR1
Target/Specificity Human COMMD1
Reconstitution & Storage PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide. Store at -20°C. Minimize freezing and thawing.
PrecautionsCOMMD1 Antibody (clone 2E2) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name COMMD1
Synonyms C2orf5, MURR1
Function Proposed scaffold protein that is implicated in diverse physiological processes and whose function may be in part linked to its ability to regulate ubiquitination of specific cellular proteins. Can modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes by displacing CAND1; in vitro promotes CRL E3 activity and dissociates CAND1 from CUL1 and CUL2 (PubMed:21778237). Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity (PubMed:15799966, PubMed:17183367, PubMed:20048074). Involved in the regulation of membrane expression and ubiquitination of SLC12A2 (PubMed:23515529). Modulates Na(+) transport in epithelial cells by regulation of apical cell surface expression of amiloride-sensitive sodium channel (ENaC) subunits and by promoting their ubiquitination presumably involving NEDD4L. Promotes the localization of SCNN1D to recycling endosomes (PubMed:14645214, PubMed:20237237, PubMed:21741370). Promotes CFTR cell surface expression through regulation of its ubiquitination (PubMed:21483833). Down-regulates SOD1 activity by interfering with its homodimerization (PubMed:20595380). Plays a role in copper ion homeostasis. Involved in copper-dependent ATP7A trafficking between the trans- Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association within the CCC complex and cooperation with the WASH complex on early endosomes (PubMed:25355947). Can bind one copper ion per monomer (PubMed:17309234). May function to facilitate biliary copper excretion within hepatocytes. Binds to phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2) (PubMed:18940794). Involved in the regulation of HIF1A-mediated transcription; competes with ARNT/Hif-1-beta for binding to HIF1A resulting in decreased DNA binding and impaired transcriptional activation by HIF-1 (PubMed:20458141).
Cellular Location Nucleus. Cytoplasm. Endosome membrane. Cytoplasmic vesicle. Early endosome Recycling endosome. Note=Shuttles between nucleus and cytosol. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins
Tissue Location Ubiquitous. Highest expression in the liver, with lower expression in brain, lung, placenta, pancreas, small intestine, heart, skeletal muscle, kidney and placenta. Down- regulated in cancer tissues.
Volume 50 µl
Research Areas
Citations (0)

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Background

Proposed scaffold protein that is implicated in diverse physiological processes and whose function may be in part linked to its ability to regulate ubiquitination of specific cellular proteins. Can modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes by displacing CAND1; in vitro promotes CRL E3 activiy and dissocoiates CAND1 from CUL1 and CUL2 (PubMed:21778237). Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity (PubMed:15799966, PubMed:17183367, PubMed:20048074). Involved in the regulation of membrane expression and ubiquitination of SLC12A2 (PubMed:23515529). Modulates Na(+) transport in epithelial cells by regulation of apical cell surface expression of amiloride-sensitive sodium channel (ENaC) subunits and by promoting their ubiquitination presumably involving NEDD4L. Promotes the localization of SCNN1D to recycling endosomes (PubMed:14645214, PubMed:20237237, PubMed:21741370). Promotes CFTR cell surface expression through regulation of its ubiquitination (PubMed:21483833). Down-regulates SOD1 activity by interfering with its homodimerization (PubMed:20595380). Plays a role in copper ion homeostasis. Involved in copper-dependent ATP7A trafficking between the trans- Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association within the CCC complex and cooperation with the WASH complex on early endosomes (PubMed:25355947). Can bind one copper ion per monomer (PubMed:17309234). May function to facilitate biliary copper excretion within hepatocytes. Binds to phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2) (PubMed:18940794). Involved in the regulation of HIF1A-mediated transcription; competes with ARNT/Hif-1-beta for binding to HIF1A resulting in decreased DNA binding and impaired transcriptional activation by HIF-1 (PubMed:20458141).

References

Mueller T.,et al.J. Hepatol. 38:164-168(2003).
Stuehler B.,et al.J. Mol. Med. 82:629-634(2004).
Zhang Z.,et al.Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).

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$ 395.00
Cat# ALS17048
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