WTAP Antibody (His351)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P |
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Primary Accession | Q15007 |
Other Accession | 9589 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 44244 Da |
Dilution | IHC-P (5 µg/ml), WB (1:500 - 1:1000), |
Gene ID | 9589 |
---|---|
Other Names | WTAP, HFL(2)D, KIAA0105, PNAS-132, WT1-associated protein, Female-lethal(2)D homolog |
Target/Specificity | Human WTAP |
Reconstitution & Storage | PBS, pH 7.2, 0.05% sodium azide. Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
Precautions | WTAP Antibody (His351) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | WTAP {ECO:0000303|PubMed:11001926, ECO:0000312|HGNC:HGNC:16846} |
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Function | Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29507755). Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724). |
Cellular Location | Nucleus speckle. Nucleus, nucleoplasm. Cytoplasm {ECO:0000250|UniProtKB:Q9ER69}. Note=Mainly nuclear with some fraction located in the cytoplasm. ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus {ECO:0000250|UniProtKB:Q9ER69} |
Tissue Location | Ubiquitously expressed. |
Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing and processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes.
References
Ortega A.,et al.J. Biol. Chem. 278:3040-3047(2003).
Nagase T.,et al.DNA Res. 2:37-43(1995).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mungall A.J.,et al.Nature 425:805-811(2003).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
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