|Application ||WB, IHC-P, IF, ICC|
|Reactivity||Human, Mouse, Rat, Pig, Bovine|
|Calculated MW||53652 Da|
|Dilution||ICC (1:1000), IF (1:1000), IHC-P (5 µg/ml), WB (1:10000),|
|Other Names||VIM, Vimentin|
|Target/Specificity||Clone 2A52 is known to react with vimentin from human, cow, pig, mouse, rat and other mammals. Since vimentin is highly conserved, it is likely that the antibody is effective on other species also.|
|Reconstitution & Storage||PBS, 10 mM sodium azide. +4°C or -20°C, Avoid repeated freezing and thawing.|
|Precautions||Vimentin Antibody (clone 2A52) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.|
|Tissue Location||Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines|
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Provided below are standard protocols that you may find useful for product applications.
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Ferrari S.,et al.Mol. Cell. Biol. 6:3614-3620(1986).
Honore B.,et al.Nucleic Acids Res. 18:6692-6692(1990).
Hartmann T.B.,et al.Br. J. Dermatol. 150:252-258(2004).
Zimbelmann R.,et al.Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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