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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Vimentin Antibody (clone 2A52)   

Vimentin Antibody (clone 2A52)

Mouse Monoclonal Antibody

     
  • IHC - Vimentin Antibody (clone 2A52) ALS17067
    Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
  • IHC - Vimentin Antibody (clone 2A52) ALS17067
    Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
  • ICC - Vimentin Antibody (clone 2A52) ALS17067
    View of mixed neuron/glial cultures stained with vimentin antibody (green) and rabbit antibody...
  • WB - Vimentin Antibody (clone 2A52) ALS17067
    Western blot of crude extract of the human carcinoma HeLa cell line.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC
Primary Accession P08670
Other Accession 7431
Reactivity Human, Mouse, Rat, Pig, Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG1,k
Clone Names 2A52
Calculated MW 53652 Da
Dilution ICC (1:1000), IF (1:1000), IHC-P (5 µg/ml), WB (1:10000),
Additional Information
Gene ID 7431
Other Names VIM, Vimentin
Target/Specificity Clone 2A52 is known to react with vimentin from human, cow, pig, mouse, rat and other mammals. Since vimentin is highly conserved, it is likely that the antibody is effective on other species also.
Reconstitution & Storage PBS, 10 mM sodium azide. +4°C or -20°C, Avoid repeated freezing and thawing.
PrecautionsVimentin Antibody (clone 2A52) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines
Research Areas
Citations (0)

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Background

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

References

Ferrari S.,et al.Mol. Cell. Biol. 6:3614-3620(1986).
Honore B.,et al.Nucleic Acids Res. 18:6692-6692(1990).
Hartmann T.B.,et al.Br. J. Dermatol. 150:252-258(2004).
Zimbelmann R.,et al.Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 395.00
Cat# ALS17067
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