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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PKM / Pyruvate Kinase Antibody   

PKM / Pyruvate Kinase Antibody

Rabbit Polyclonal Antibody

     
  • IHC - PKM / Pyruvate Kinase Antibody ALS17073
    Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
  • IHC - PKM / Pyruvate Kinase Antibody ALS17073
    Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
  • IHC - PKM / Pyruvate Kinase Antibody ALS17073
    Immunohistochemistry of paraffin-embedded rat kidney using PKM2 antibody at dilution of 1:100...
  • IHC - PKM / Pyruvate Kinase Antibody ALS17073
    Immunohistochemistry of paraffin-embedded mouse lung using PKM2 antibody at dilution of 1:100...
  • IF - PKM / Pyruvate Kinase Antibody ALS17073
    Immunofluorescence analysis of HeLa cell using PKM2 antibody. Blue: DAPI for nuclear staining.
  • WB - PKM / Pyruvate Kinase Antibody ALS17073
    Western blot analysis of extracts of various cell lines, using PKM2 antibody.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IHC-P, IF
Primary Accession P14618
Other Accession 5315
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 57937 Da
Dilution IF (1:20 - 1:100), IHC (1:50 - 1:200), IHC-P (10 µg/ml), WB (1:500 - 1:2000),
Additional Information
Gene ID 5315
Other Names PKM, p58, PK3, PKM2, Pyruvate Kinase, Pyruvate kinase 2/3, Pyruvate kinase, m1, Pyruvate kinase, muscle, TCB, Tumor M2-PK, OIP-3, OIP3, Opa-interacting protein 3, CTHBP, PK, muscle type, PK2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle iso ...
Target/Specificity Human PKM / Pyruvate Kinasex
Reconstitution & Storage PBS, pH 7.3, 0.02% sodium azide, 50% glycerol. Long term: -80°C; Short term: -20°C. Avoid freeze-thaw cycles.
PrecautionsPKM / Pyruvate Kinase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PKM
Synonyms OIP3, PK2, PK3, PKM2
Function Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Cellular Location Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity
Tissue Location Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
Volume 50 µl
Research Areas
Citations (0)

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Background

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

References

Tani K.,et al.Gene 73:509-516(1988).
Kato H.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:7861-7865(1989).
Kato H.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:1625-1625(1990).
Takenaka M.,et al.Eur. J. Biochem. 198:101-106(1991).
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 395.00
Cat# ALS17073
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