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AIRE Antibody

Rabbit Polyclonal Antibody

     
  • IHC - AIRE Antibody ALS17077
    Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
  • IF - AIRE Antibody ALS17077
    Immunofluorescence analysis of A549 cell using AIRE antibody. Blue: DAPI for nuclear staining.
  • WB - AIRE Antibody ALS17077
    Western blot analysis of extracts of various cell lines, using AIRE antibody.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession O43918
Other Accession 326
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 57727 Da
Dilution IF (1:50 - 1:200), IHC-P (5 µg/ml), WB (1:500 - 1:2000),
Additional Information
Gene ID 326
Other Names AIRE, APSI, AIRE1, Autoimmune regulator, APECED, APS1, APECED protein, PGA1
Target/Specificity Human AIRE
Reconstitution & Storage PBS, pH 7.3, 0.02% sodium azide, 50% glycerol. Long term: -80°C; Short term: -20°C. Avoid freeze-thaw cycles.
PrecautionsAIRE Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AIRE
Synonyms APECED
Function Transcriptional regulator that binds to DNA as a dimer or as a tetramer, but not as a monomer. Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes (By similarity). Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Functions as a transcriptional activator and promotes the expression of otherwise tissue-specific self-antigens in the thymus, which is important for self tolerance and the avoidance of autoimmune reactions.
Cellular Location Nucleus. Cytoplasm. Note=Associated with tubular structures and in discrete nuclear dots resembling ND10 nuclear bodies. May shuttle between nucleus and cytoplasm
Tissue Location Widely expressed. Expressed at higher level in thymus (medullary epithelial cells and monocyte-dendritic cells), pancreas, adrenal cortex and testis. Expressed at lower level in the spleen, fetal liver and lymph nodes. Isoform 2 and isoform 3 seem to be less frequently expressed than isoform 1, if at all
Volume 50 µl
Research Areas
Citations (0)

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Background

Transcriptional regulator that binds to DNA as a dimer or as a tetramer, but not as a monomer. Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes (By similarity). Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Functions as a transcriptional activator and promotes the expression of otherwise tissue-specific self-antigens in the thymus, which is important for self tolerance and the avoidance of autoimmune reactions.

References

Nagamine K.,et al.Nat. Genet. 17:393-398(1997).
Aaltonen J.,et al.Nat. Genet. 17:399-403(1997).
Lee Y.S.,et al.Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
Hattori M.,et al.Nature 405:311-319(2000).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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$ 395.00
Cat# ALS17077
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