|Application ||WB, IHC-P, IF, E|
|Calculated MW||122218 Da|
|Dilution||IF (20 µg/ml), IHC-P (10 µg/ml), WB (1 - 2 µg/ml),|
|Other Names||USP25, Deubiquitinating enzyme 25, Ubiquitin thiolesterase 25, Ubiquitin thioesterase 25, USP on chromosome 21, Ubiquitin specific protease 25|
|Target/Specificity||USP25 antibody is human and mouse reactive. Multiple isoforms of USP25 are known to exist.|
|Reconstitution & Storage||PBS, 0.02% sodium azide. Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||USP25 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.|
|Tissue Location||Isoform USP25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific.|
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Provided below are standard protocols that you may find useful for product applications.
Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
Valero R.,et al.Genomics 62:395-405(1999).
Groet J.,et al.Genes Chromosomes Cancer 27:153-161(2000).
Valero R.,et al.Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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