|Application ||WB, IHC-P, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||108314 Da|
|Dilution||IHC-P (10 µg/ml), WB (1 - 2 µg/ml),|
|Other Names||IQSEC1, ARF-GEP100, ARFGEP100, Brefeldin A-resistant ARF-GEF2, BRAG2, IQ motif and Sec7 domain 1, KIAA0763, GEP100|
|Target/Specificity||IQSEC1 antibody is human, mouse and rat reactive. At least four isoforms of IQSEC1 are known to exist. IQSEC1 antibody is predicted to not cross-react with IQSEC2.|
|Reconstitution & Storage||PBS, 0.02% sodium azide. Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||IQSEC1 Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ARFGEP100, BRAG2, KIAA0763|
|Function||Guanine nucleotide exchange factor for ARF1 and ARF6 (PubMed:24058294). Guanine nucleotide exchange factor activity is enhanced by lipid binding (PubMed:24058294). Accelerates GTP binding by ARFs of all three classes. Guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.|
|Cellular Location||Cytoplasm. Nucleus. Note=At steady state, may be preferentially cytosolic|
|Tissue Location||Expressed in brain, ovary, heart, lung, liver, kidney and leukocytes. Moderate expression was also detected in lung, skeletal muscle, placenta, small intestine, pancreas, spleen and testis.|
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Provided below are standard protocols that you may find useful for product applications.
In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.
Dunphy J.L.,et al.Curr. Biol. 16:315-320(2006).
Nagase T.,et al.DNA Res. 5:277-286(1998).
Someya A.,et al.Proc. Natl. Acad. Sci. U.S.A. 98:2413-2418(2001).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Mayya V.,et al.Sci. Signal. 2:RA46-RA46(2009).
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