|Application ||WB, IHC-P, E|
|Calculated MW||31122 Da|
|Dilution||IHC-P (10 µg/ml),|
|Other Names||EEF1D, Antigen NY-CO-4, EF-1D, EF-1-delta, Elongation factor 1-delta, FP1047, EF1D|
|Reconstitution & Storage||PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||EEF1D Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1- alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.|
|Cellular Location||Isoform 2: Nucleus|
|Tissue Location||Isoform 2 is specifically expressed in brain, cerebellum and testis|
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Provided below are standard protocols that you may find useful for product applications.
Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1- alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.
Sanders J.P.,et al.Biochim. Biophys. Acta 1174:87-90(1993).
Scanlan M.J.,et al.Int. J. Cancer 76:652-658(1998).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Nusbaum C.,et al.Nature 439:331-335(2006).
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