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CYP24 / CYP24A1 Antibody (aa400-450)

Rabbit Polyclonal Antibody

     
  • IHC-P - CYP24 / CYP24A1 Antibody (aa400-450) ALS17204
    Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
    detail
  • IHC-P - CYP24 / CYP24A1 Antibody (aa400-450) ALS17204
    Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
    detail
  • WB - CYP24 / CYP24A1 Antibody (aa400-450) ALS17204
    Western blot (WB) analysis of Anti-CYP24A1 pAb at 1:500 dilution.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q07973
Other Accession 1591
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 58875 Da
Dilution IHC-P (5 µg/ml), WB (1:500 - 1:1000),
Additional Information
Gene ID 1591
Other Names CYP24A1, 24-OHase, CP24, CYP24, Cytochrome P450-CC24, Exo-mitochondrial protein, HCAI, p450-CC24, Vitamin D 24-hydroxylase, Cytochrome P450 24A1, Vitamin D(3) 24-hydroxylase
Target/Specificity Human CYP24 / CYP24A1
Reconstitution & Storage PBS, pH 7.2, 0.05% sodium azide. Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
PrecautionsCYP24 / CYP24A1 Antibody (aa400-450) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYP24A1 (HGNC:2602)
Synonyms CYP24
Function A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)) (PubMed:24893882, PubMed:15574355, PubMed:8679605, PubMed:11012668, PubMed:16617161, PubMed:29461981). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid (PubMed:24893882, PubMed:15574355). With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product (PubMed:11012668, PubMed:8679605). Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S- hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol (PubMed:25727742). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed:8679605).
Cellular Location Mitochondrion {ECO:0000250|UniProtKB:Q09128}.
Volume 50 µl
Research Areas
Citations (0)
citation

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Background

Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product.

References

Chen K.-S.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:4543-4547(1993).
Ren S.,et al.J. Biol. Chem. 280:20604-20611(2005).
Deloukas P.,et al.Nature 414:865-871(2001).
Chen K.-S.,et al.Biochim. Biophys. Acta 1263:1-9(1995).
Labuda M.,et al.J. Bone Miner. Res. 8:1397-1406(1993).

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Discontinued
Cat# ALS17204
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