|Application ||WB, IHC-P, ICC, E, IP|
|Calculated MW||141840 Da|
|Dilution||IHC-P (10 µg/ml), IP (1:1000 - 1:2000), IP (1:1000 - 1:2000), WB (1:1000 - 1:2000),|
|Other Names||APAF1, APAF-1, CED4, KIAA0413|
|Target/Specificity||Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.|
|Reconstitution & Storage||PBS, 0.02% sodium azide. Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.|
|Precautions||APAF1 / APAF-1 Antibody (aa1-464, clone 2E12, Low Endotoxin) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.|
|Tissue Location||Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver|
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Provided below are standard protocols that you may find useful for product applications.
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Zou H.,et al.Cell 90:405-413(1997).
Hahn C.,et al.Biochem. Biophys. Res. Commun. 261:746-749(1999).
Saleh A.,et al.J. Biol. Chem. 274:17941-17945(1999).
Hu Y.,et al.EMBO J. 18:3586-3595(1999).
Ogawa T.,et al.Biochem. Biophys. Res. Commun. 306:537-543(2003).
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