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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   DDIT3 / CHOP Antibody   

DDIT3 / CHOP Antibody

Rabbit Polyclonal Antibody

     
  • IHC-P - DDIT3 / CHOP Antibody ALS17237
    Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
  • IF - DDIT3 / CHOP Antibody ALS17237
    Immunofluorescence analysis of A549 cell using DDIT3 antibody. Blue: DAPI for nuclear staining.
  • WB - DDIT3 / CHOP Antibody ALS17237
    Western blot analysis of extracts from mouse craniofacial tissue, using DDIT3 antibody.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession P35638
Other Accession 1649
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 19175 Da
Dilution IF (1:50 - 1:200), IHC-P (5 µg/ml), WB (1:500 - 1:2000),
Additional Information
Gene ID 1649
Other Names DDIT3, CHOP10, DDIT-3, CHOP, GADD153, C/EBP zeta, C/EBP-homologous protein, C/EBP-homologous protein 10, CHOP-10
Target/Specificity Human DDIT3 / CHOP
Reconstitution & Storage PBS, pH 7.3, 0.02% sodium azide, 50% glycerol. Long term: -80°C; Short term: -20°C. Avoid freeze-thaw cycles.
PrecautionsDDIT3 / CHOP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DDIT3
Synonyms CHOP, CHOP10, GADD153
Function Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response.
Cellular Location Cytoplasm. Nucleus. Note=Present in the cytoplasm under non-stressed conditions and ER stress leads to its nuclear accumulation
Volume 50 µl
Research Areas
Citations (0)

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Background

Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response.

References

Park J.S.,et al.Gene 116:259-267(1992).
Crozat A.,et al.Nature 363:640-644(1993).
Rabbitts T.H.,et al.Nat. Genet. 4:175-180(1993).
Li X.,et al.Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
Scherer S.E.,et al.Nature 440:346-351(2006).

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$ 395.00
Cat# ALS17237
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