CDC20 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF |
---|---|
Primary Accession | Q12834 |
Other Accession | 991 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 54723 Da |
Dilution | IF (1:50 - 1:200), IHC-P (5 µg/ml), WB (1:500 - 1:2000), |
Gene ID | 991 |
---|---|
Other Names | CDC20, BA276H19.3, CDC20A, p55CDC |
Target/Specificity | Human CDC20 |
Reconstitution & Storage | PBS, pH 7.3, 0.02% sodium azide, 50% glycerol. Long term: -80°C; Short term: -20°C. Avoid freeze-thaw cycles. |
Precautions | CDC20 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CDC20 |
---|---|
Function | Involved in the metaphase/anaphase transition of cell cycle (PubMed:32666501). Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. The CDC20-APC/C complex promotes proper dilation formation and radial migration by degrading CCDC41 (By similarity). |
Cellular Location | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole |
Volume | 50 µl |
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Background
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.
References
Weinstein J.,et al.Mol. Cell. Biol. 14:3350-3363(1994).
Kramer E.R.,et al.Curr. Biol. 8:1207-1210(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Gregory S.G.,et al.Nature 441:315-321(2006).
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