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PSMB5 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF |
|---|---|
| Primary Accession | P28074 |
| Other Accession | 5693 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 28480 Da |
| Dilution | IF (1:50 - 1:200), IHC-P (2.5 µg/ml), WB (1:500 - 1:2000), |
| Gene ID | 5693 |
|---|---|
| Other Names | PSMB5, Proteasome chain 6, Proteasome subunit MB1, Proteasome subunit X, Proteasome catalytic subunit 3, Macropain epsilon chain, MB1, LMPX, Proteasome beta 5 subunit, Proteasome epsilon chain, Proteasome subunit beta type-5 |
| Target/Specificity | Human PSMB5 / MB1. |
| Reconstitution & Storage | PBS, pH 7.3, 0.02% sodium azide, 50% glycerol. Long term: -80°C; Short term: -20°C. Avoid freeze-thaw cycles. |
| Precautions | PSMB5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PSMB5 |
|---|---|
| Synonyms | LMPX, MB1, X |
| Function | Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP- dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin- independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB5 displays a chymotrypsin-like activity. |
| Cellular Location | Cytoplasm. Nucleus. Note=Translocated from the cytoplasm into the nucleus following interaction with AKIRIN2, which bridges the proteasome with the nuclear import receptor IPO9 |
| Volume | 50 µl |
Provided below are standard protocols that you may find useful for product applications.
Background
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage through the Nrf2-ARE pathway (By similarity).
References
Abdulla S.,et al.Immunogenetics 44:254-258(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Heilig R.,et al.Nature 421:601-607(2003).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
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