|Application ||WB, IHC-P, IF|
|Calculated MW||141133 Da|
|Dilution||IF (1:100), IHC-P (10 µg/ml), WB (1:1000)|
|Other Names||CLASP2, CLIP-associating protein 2, Protein Orbit homolog 2, HOrbit2, KIAA0627|
|Reconstitution & Storage||PBS, 0.1% sodium azide. Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||Anti-CLASP2 Antibody (N-Terminus, clone KT69) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.|
|Cellular Location||Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Golgi apparatus. Golgi apparatus, trans-Golgi network Cell membrane. Cell projection, ruffle membrane. Note=Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane|
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