|Application ||WB, IHC-P, IF|
|Predicted||Human, Mouse, Rat, Monkey, Dog|
|Calculated MW||23490 Da|
|Dilution||IF (1:50 - 1:250), IHC-P (5 µg/ml), WB (1:250 - 1:2000)|
|Other Names||RAB7A, CMT2B, PRO2706, RAB7, Ras-associated protein RAB7, Ras-related protein Rab-7a, PSN|
|Target/Specificity||Detects Rab7a protein in the human, rat and mouse whole cell lysates and transfected cells with GFP-Rab7a by Western blot. This Ab is specific for Rab7a.|
|Reconstitution & Storage||PBS, 20% glycerol, 0.05% sodium azide. Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.|
|Precautions||Anti-RAB7A / RAB7 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient- transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation.|
|Cellular Location||Late endosome. Lysosome. Cytoplasmic vesicle, phagosome. Melanosome. Cytoplasmic vesicle, phagosome membrane; Lipid- anchor; Cytoplasmic side. Note=Colocalizes with OSBPL1A at the late endosome. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts Recruited to phagosomes containing S.aureus or Mycobacterium (By similarity).|
|Tissue Location||Widely expressed; high expression found in skeletal muscle.|
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