|Application ||WB, IHC-P|
|Predicted||Human, Mouse, Rat|
|Calculated MW||112694 Da|
|Dilution||IHC-P (1:100), WB (1:500 - 1:1000)|
|Other Names||ULK2, ATG1B, KIAA0623, Unc-51-like kinase 2, Unc51.2|
|Target/Specificity||Recognizes endogenous levels of ULK2 protein.|
|Reconstitution & Storage||PBS, pH 7.3, 0.01% sodium azide, 30% glycerol. Store at -20°C. Aliquot to avoid freeze/thaw cycles.|
|Precautions||Anti-ULK2 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons.|
|Cellular Location||Cytoplasmic vesicle membrane; Peripheral membrane protein. Note=Localizes to pre- autophagosomal membrane|
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