|Application ||WB, IHC-P|
|Calculated MW||179350 Da|
|Dilution||IHC-P (1:150, 10 µg/ml), WB (1:2000)|
|Other Names||TET3, HCG_40738, KIAA0401|
|Reconstitution & Storage||PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide Store at -20°C. Minimize freezing and thawing.|
|Precautions||Anti-TET3 Antibody (aa241-568, clone 11E11) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5- hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin (By similarity). In addition to its role in DNA demethylation, also involved in the recruitment of the O- GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.|
|Cellular Location||Nucleus. Cytoplasm. Note=At the zygotic stage, localizes in the male pronucleus, while it localizes to the cytoplasm at other preimplantation stages.|
|Tissue Location||Expressed in colon, muscle, adrenal gland and peripheral blood lymphocytes.|
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