|Application ||WB, IHC-P|
|Predicted||Human, Mouse, Rat|
|Calculated MW||33067 Da|
|Dilution||IHC-P (5 µg/ml), WB (1:500 - 1:2000)|
|Other Names||CCND2, KIAK0002, G1/S-specific cyclin D2, G1/S-specific cyclin-D2, Cyclin D2|
|Target/Specificity||Human CCND2 / Cyclin D2|
|Reconstitution & Storage||PBS, 0.1% sodium azide, 50% glycerol. Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
|Precautions||Anti-CCND2 / Cyclin D2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).|
|Cellular Location||Nucleus. Cytoplasm. Membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members|
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