|Application ||WB, IHC-P|
|Predicted||Human, Mouse, Rat|
|Calculated MW||24824 Da|
|Dilution||IHC-P (10 µg/ml), WB|
|Other Names||UCHL1, Ubiquitin C-terminal hydrolase, PARK5, PGP9.5, PGP95, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Uch-L1|
|Target/Specificity||Human UCHL1 / PGP9.5|
|Reconstitution & Storage||PBS, 0.05% sodium azide. Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
|Precautions||Anti-UCHL1 / PGP9.5 Antibody (clone 31A3) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.|
|Cellular Location||Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain|
|Tissue Location||Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients|
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