|Application ||WB, IHC-P, IF, ICC|
|Predicted||Human, Mouse, Monkey, Bovine|
|Calculated MW||103932 Da|
|Dilution||ICC (1:100 - 1:500), IF, IHC-P (1:100), WB (1:500 - 1:1000)|
|Other Names||PKN1, PKN, Protein kinase C-like PKN, Protein kinase N1, PRK1, PRKCL1, Serine-threonine kinase N, Pknalpha, Protease-activated kinase 1, Protein kinase PKN-alpha, DBK, PAK-1, PKN-ALPHA, Protein kinase C-like 1|
|Target/Specificity||Recognizes endogenous levels of PKN1 protein.|
|Reconstitution & Storage||PBS, pH 7.3, 0.01% sodium azide, 30% glycerol. Store at -20°C. Aliquot to avoid freeze/thaw cycles.|
|Precautions||Anti-PKN1 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||PAK1, PKN, PRK1, PRKCL1|
|Function||PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser- 159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.|
|Cellular Location||Cytoplasm. Nucleus. Endosome. Cell membrane; Peripheral membrane protein. Cleavage furrow. Midbody Note=Associates with chromatin in a ligand-dependent manner Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-374 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis|
|Tissue Location||Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells|
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