|Application ||WB, IHC-P, IP|
|Predicted||Human, Mouse, Rat|
|Calculated MW||121978 Da|
|Dilution||IHC-P (5 µg/ml), IP (20 µg/ml), WB (0.5 - 4 µg/ml)|
|Other Names||HDAC5, Antigen NY-CO-9, Histone deacetylase 5, NY-CO-9, HD5, KIAA0600|
|Target/Specificity||The antibody detects ~124 kD histone deacetylase 5. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, and 8.|
|Reconstitution & Storage||Affinity purified|
|Precautions||Anti-HDAC5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.|
|Cellular Location||Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by AMPK, CaMK1 and SIK1|
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