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> home > Products > Primary Antibodies > Antibody Collections > GPCR Antibodies > Anti-YBX1 / YB1 Antibody (clone 4F12)
Anti-YBX1 / YB1 Antibody (clone 4F12)
Mouse Anti Human Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | P67809 |
| Predicted | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a,k |
| Clone Names | 4F12 |
| Calculated MW | 35924 Da |
| Dilution | ELISA, IF (20 µg/ml), IHC-P (10 µg/ml), WB |
| Gene ID | 4904 |
|---|---|
| Alias Symbol | YBX1 |
| Other Names | YBX1, CBF-A, DBPB, CSDB, DNA-binding protein B, MDR-NF1, NSEP-1, NSEP1, Y box binding protein 1, Y-box transcription factor, Y-box-binding protein 1, YB-1, BP-8, CSDA2, EFI-A, Enhancer factor I subunit A, YB1 |
| Target/Specificity | Human YBX1 / YB1 |
| Reconstitution & Storage | Protein A purified |
| Precautions | Anti-YBX1 / YB1 Antibody (clone 4F12) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | YBX1 (HGNC:8014) |
|---|---|
| Function | DNA- and RNA-binding protein involved in various processes, such as translational repression, RNA stabilization, mRNA splicing, DNA repair and transcription regulation (PubMed:8188694, PubMed:10817758, PubMed:11698476, PubMed:14718551, PubMed:18809583, PubMed:31358969). Predominantly acts as a RNA-binding protein: binds preferentially to the 5'-[CU]CUGCG-3' RNA motif and specifically recognizes mRNA transcripts modified by C5-methylcytosine (m5C) (PubMed:19561594, PubMed:31358969). Promotes mRNA stabilization: acts by binding to m5C- containing mRNAs and recruiting the mRNA stability maintainer ELAVL1, thereby preventing mRNA decay (PubMed:10817758, PubMed:11698476, PubMed:31358969). Component of the CRD-mediated complex that promotes MYC mRNA stability (PubMed:19029303). Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Plays a key role in RNA composition of extracellular exosomes by defining the sorting of small non-coding RNAs, such as tRNAs, Y RNAs, Vault RNAs and miRNAs (PubMed:27559612, PubMed:29073095). Probably sorts RNAs in exosomes by recognizing and binding C5-methylcytosine (m5C)-containing RNAs (PubMed:28341602, PubMed:29073095). Acts as a key effector of epidermal progenitors by preventing epidermal progenitor senescence: acts by regulating the translation of a senescence-associated subset of cytokine mRNAs, possibly by binding to m5C-containing mRNAs (PubMed:29712925). Also involved in pre-mRNA alternative splicing regulation: binds to splice sites in pre-mRNA and regulates splice site selection (PubMed:12604611). Binds to TSC22D1 transcripts, thereby inhibiting their translation and negatively regulating TGF-beta- mediated transcription of COL1A2 (By similarity). Also able to bind DNA: regulates transcription of the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys- 7' (PubMed:18809583). Binds to promoters that contain a Y-box (5'- CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes (PubMed:8188694, PubMed:18809583). Promotes separation of DNA strands that contain mismatches or are modified by cisplatin (PubMed:14718551). Has endonucleolytic activity and can introduce nicks or breaks into double- stranded DNA, suggesting a role in DNA repair (PubMed:14718551). The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation (PubMed:19483673). |
| Cellular Location | Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Secreted, extracellular exosome. Cytoplasm, P-body {ECO:0000250|UniProtKB:P62960}. Note=Predominantly cytoplasmic in proliferating cells (PubMed:12604611). Cytotoxic stress and DNA damage enhance translocation to the nucleus (PubMed:14718551) Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:25229427). Shuttles between nucleus and cytoplasm (PubMed:25229427). Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress (PubMed:18335541). Secreted by mesangial and monocytic cells after inflammatory challenges (PubMed:19483673) |
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$ 533.50
Cat# ALS17987
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