|Application ||WB, IHC-P, E|
|Calculated MW||27042 Da|
|Dilution||ELISA (1:128000), IHC-P (5 µg/ml), WB (0.1 - 0.3 µg/ml)|
|Other Names||VEGFA, VPF, Vascular permeability factor, VEGF, VEGF-A, MVCD1|
|Target/Specificity||Human VEGFA / VEGF. This antibody is expected to recognize all reported isoforms.|
|Reconstitution & Storage||Immunoaffinity purified|
|Precautions||Anti-VEGFA / VEGF Antibody (aa288-299) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.|
|Cellular Location||Secreted Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin|
|Tissue Location||Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland|
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