|Application ||WB, IHC-P, E, IP|
|Predicted||Human, Mouse, Rat, Monkey, Bovine|
|Calculated MW||26266 Da|
|Dilution||ELISA, IHC-P (1:100), IP (1:200), WB (1:1000)|
|Other Names||BCL2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, B-cell lymphoma protein 2, Bcl-2, PPP1R50|
|Target/Specificity||Detects endogenous levels of total human Bcl-2 protein at 26kD. This antibody does not cross-react with other Bcl-2 family members. Species cross-reactivity: mouse, rat. Species sequence homology: monkey and bovine. Antibody fails in chicken despite ...|
|Reconstitution & Storage||Tissue culture supernatant|
|Precautions||Anti-BCL2 / Bcl-2 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).|
|Cellular Location||Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein|
|Tissue Location||Expressed in a variety of tissues.|
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