|Application ||WB, IHC-P, IF, ICC|
|Predicted||Human, Mouse, Rat, Pig, Bovine|
|Calculated MW||36053 Da|
|Dilution||ICC (1:100), IF (1:100), IHC-P (1:50), WB (1:1000)|
|Other Names||GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit|
|Target/Specificity||Clone 1D4 recognizes GAPDH specifically both in western blots and in immunocytochemical experiments. On blots clone 1D4 reveals a prominent ~38kDa band, and on cells in tissue culture the antibody stains in a punctate cytoplasmic fashion. clone 1D4 i ...|
|Reconstitution & Storage||Tissue culture supernatant|
|Precautions||Anti-GAPDH Antibody (clone 1D4) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.|
|Cellular Location||Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton. Note=Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.|
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