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> home > Products > Primary Antibodies > Antibody Collections > GPCR Antibodies > Anti-GAPDH Antibody (clone 1D4)
Anti-GAPDH Antibody (clone 1D4)
Mouse Anti Human Monoclonal Antibody
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Application 
| WB, IHC-P, IF, ICC |
|---|---|
| Primary Accession | P04406 |
| Predicted | Human, Mouse, Rat, Pig, Bovine |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgM,k |
| Clone Names | 1D4 |
| Calculated MW | 36053 Da |
| Dilution | ICC (1:100), IF (1:100), IHC-P (1:50), WB (1:1000) |
| Gene ID | 2597 |
|---|---|
| Alias Symbol | GAPDH |
| Other Names | GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit |
| Target/Specificity | Clone 1D4 recognizes GAPDH specifically both in western blots and in immunocytochemical experiments. On blots clone 1D4 reveals a prominent ~38kDa band, and on cells in tissue culture the antibody stains in a punctate cytoplasmic fashion. clone 1D4 i ... |
| Reconstitution & Storage | Tissue culture supernatant |
| Precautions | Anti-GAPDH Antibody (clone 1D4) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GAPDH {ECO:0000303|PubMed:2987855, ECO:0000312|HGNC:HGNC:4141} |
|---|---|
| Function | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:3170585, PubMed:11724794). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D- glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:3170585, PubMed:11724794). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1- dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). |
| Cellular Location | Cytoplasm, cytosol. Nucleus {ECO:0000250|UniProtKB:P04797}. Cytoplasm, perinuclear region. Membrane Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P04797} Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions (PubMed:12829261) {ECO:0000250|UniProtKB:P04797, ECO:0000269|PubMed:12829261} |
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