|Application ||WB, IHC-P, E|
|Calculated MW||17999 Da|
|Dilution||ELISA (1:20000 - 1:80000), IHC-P (5 µg/ml), WB (1:500 - 1:1000)|
|Other Names||ISCU, ISU2, NifU-like protein, NIFUN, HML, NIFU, HnifU|
|Target/Specificity||Human HML / ISCU.|
|Reconstitution & Storage||Affinity purified|
|Precautions||Anti-ISCU Antibody (aa3-148) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Scaffold protein for the de novo synthesis of iron- sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins (PubMed:11060020). First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH (By similarity).|
|Cellular Location||Isoform 1: Mitochondrion|
|Tissue Location||Detected in heart, liver, skeletal muscle, brain, pancreas, kidney, lung and placenta|
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