|Application ||WB, IHC-P|
|Calculated MW||42593 Da|
|Dilution||IHC-P (10 µg/ml), WB (0.5 - 1 µg/ml)|
|Other Names||FEN1, DNase IV, FEN-1, HFEN-1, Flap endonuclease 1, MF1, RAD2, Maturation factor 1, Maturation factor-1|
|Target/Specificity||Endogenous levels of human FEN-1 protein. Positive Control: HeLa, HepG2 and HEK293.|
|Reconstitution & Storage||Immunoaffinity purified|
|Precautions||Anti-FEN1 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.|
|Cellular Location||Isoform 1: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage|
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