|Application ||WB, IHC-P, Flo|
|Calculated MW||73913 Da|
|Dilution||Flo (1 - 3 µg/10E6 cells), IHC-P (10 µg/ml), WB (0.5 - 1 µg/ml)|
|Other Names||NDC80, HEC1, Kinetochore protein Hec1, Kinetochore associated 2, HEC, HsHec1, HsNDC80, KNTC2, TID3|
|Target/Specificity||Endogenous levels of human HEC1.|
|Reconstitution & Storage||Immunoaffinity purified|
|Precautions||Anti-HEC1 / NDC80 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||HEC, HEC1, KNTC2|
|Function||Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020). Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed:25743205, PubMed:23891108).|
|Cellular Location||Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore|
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