|Application ||WB, IHC-P, E|
|Calculated MW||35864 Da|
|Dilution||ELISA, IHC-P (10 µg/ml), WB|
|Other Names||ATG3, APG3-LIKE, APG3, APG3L, Autophagy related 3, HApg3, PC3-96, Protein PC3-96, Autophagy-related protein 3|
|Reconstitution & Storage||Caprylic acid and ammonium sulfate precipitation|
|Precautions||Anti-ATG3 Antibody (aa1-314) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12- ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.|
|Tissue Location||Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta|
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