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HA Tag Antibody

Mouse Monoclonal Antibody (Mab)

     
  • WB - HA Tag Antibody AM1008a
    HA-tagged recombinant protein was expressed in COS7 cells. 15 μg of cell lysate was loaded onto a gel to test the HA tag antibody (AM1008a).
  • WB - HA Tag Antibody AM1008a
    Western blot analysis of anti-HA Tag Antibody (AM1008a) in 12tag recombinant protein. 12tag recombinant protein was detected using the purified Mab.
  • SPECIFICATION
  • CITATIONS: 22
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG2b
Clone Names 12CA5
Additional Information
Other Names Tag from influenza hemagglutinin protein
Target/Specificity KLH conjugated synthetic peptide encoding HA tag (YPYDVPDYA) was used as antigen.
Dilution WB~~1:1,000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHA Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations ( 0 )

Background

Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.

References

Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. Sells MA and Chernoff J. (1995) Gene, 152:187-9.

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$ 295.00
$ 99.00
Cat# AM1008a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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