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GFP Tag Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • WB - GFP Tag Antibody AM1009a
    Western blot analysis of lysate from GFP protein, using GFP Tag Antibody(Cat. #AM1009a). AM1009a was diluted at 1:4000. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.
  • IF - GFP Tag Antibody AM1009a
    Immuno-fluorescence for GFP Tag Antibody (Cat # AM1009A). Antibody target is a YFP tagged protein (the inner membrane complex) of Toxoplasma gondii, which localizes around the exterior of the parasite. Brighter parasites represent actively dividing cells and darker ones are not actively dividing. Secondary goat-anti-mouse and goat-anti-rabbit were conjugated with Alexa546 (Molecular Probes)at 1:200 dilution. Phase contrast enhancement applied. Fluorescent lamp exposure times as follows: RFP Channel, 0.075 sec; YFP Channel, 1.000 sec; DAPI Channel, 0.020 sec; Phase Channel: 0.300 sec. Microscope: Axiovert 200M inverted microscope (Zeiss) equipped with a PRIOR stage and a NEOFLUAR 100x/1.3 objective lens. Images were collected with a Hamamatsu C4742-95 CCD camera. Chroma Filtersets: TRITC, HQ545/30x – Q570LP – HQ620/60m; DAPI, D350/50x – E420LP – 400DCLP; YFP, Q151LP – HQ500/20x – HQ535/30m. Data courtesy of Seth Robertson, Boston College.
  • IHC - GFP Tag Antibody AM1009a
    Formalin-fixed and paraffin-embedded mouse skin tissue expression GFP reacted with GFP Tag Antibody (Cat.#AM1009a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. -ve shows negative staining of the antibody in a wild type mouse, whereas +ve shows positive staining in a mouse expressing GFP under the LGR5 promoter. The staining shows localization of GFP at the bottom of the hair follicle. The antigen was retrieved in citrate pH 6 in water bath for 25 mins.
  • SPECIFICATION
  • CITATIONS: 15
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  • BACKGROUND
Product Information
ApplicationWB, IHC, IF, E
ReactivityHuman
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1
Clone Names168AT1211
Calculated MW27 k KDa
Additional Information
Target/SpecificityPurified His-tagged GFP protein was used to produced this monoclonal antibody.
DilutionWB~~1:4000
IF~~1:50~100
IHC~~1:50~100
FormatPurified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Protein Information
Research Areas
Citations ( 0 )

Background

Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. Abgent's anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Abgent's anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.

References

Ward, W. W., et al.(1980) Photochem. Photobiol. 31:611

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$ 195.00
$ 95.00
Cat# AM1009a
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(40 western blots)
Availability: In Stock
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