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GFP Tag Antibody

Purified Mouse Monoclonal Antibody (Mab)

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United States
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Ordering Information
Catalog # SizeAvailability Price  
AM1009a 400 µl
(40 western blots)
In Stock
$ 195.00email & fax$ 185.25online
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AM1009a-ev 80 µl
(8 western blots)
In Stock
$ 95.00email & fax$ 90.25online
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  • Specification
  • Citations : 9
  • Reviews
  • Protocols
  • Background

GFP Tag Antibody - Product info

Application
  • Applications Legend:
  • WB=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC
IsotypeMouse IgG1
Clone Names168AT1211
Calculated MW27 k Da

GFP Tag Antibody - Additional info

Target/Specificity
Purified His-tagged GFP protein was used to produced this monoclonal antibody.
Dilution
WB~~1:1000
IF~~1:50~100
IHC~~1:50~100
Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
GFP Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

GFP Tag Antibody - Research Areas

Application data

  • WB - GFP Tag Antibody AM1009a

    Western blot analysis of lysate from GFP protein, using GFP Tag Antibody(Cat. #AM1009a). AM1009a was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug.

  • IF - GFP Tag Antibody AM1009a

    Immuno-fluorescence for GFP Tag Antibody (Cat # AM1009A). Antibody target is a YFP tagged protein (the inner membrane complex) of Toxoplasma gondii, which localizes around the exterior of the parasite. Brighter parasites represent actively dividing cells and darker ones are not actively dividing. Secondary goat-anti-mouse and goat-anti-rabbit were conjugated with Alexa546 (Molecular Probes)at 1:200 dilution. Phase contrast enhancement applied. Fluorescent lamp exposure times as follows: RFP Channel, 0.075 sec; YFP Channel, 1.000 sec; DAPI Channel, 0.020 sec; Phase Channel: 0.300 sec. Microscope: Axiovert 200M inverted microscope (Zeiss) equipped with a PRIOR stage and a NEOFLUAR 100x/1.3 objective lens. Images were collected with a Hamamatsu C4742-95 CCD camera. Chroma Filtersets: TRITC, HQ545/30x – Q570LP – HQ620/60m; DAPI, D350/50x – E420LP – 400DCLP; YFP, Q151LP – HQ500/20x – HQ535/30m. Data courtesy of Seth Robertson, Boston College.

  • IHC - GFP Tag Antibody AM1009a

    Formalin-fixed and paraffin-embedded mouse skin tissue expression GFP reacted with GFP Tag Antibody (Cat.#AM1009a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. -ve shows negative staining of the antibody in a wild type mouse, whereas +ve shows positive staining in a mouse expressing GFP under the LGR5 promoter. The staining shows localization of GFP at the bottom of the hair follicle. The antigen was retrieved in citrate pH 6 in water bath for 25 mins.

  • WB - GFP Tag Antibody AM1009a
  • IF - GFP Tag Antibody AM1009a
  • IHC - GFP Tag Antibody AM1009a

ELITE™ Custom Antibody Services

  • RpL22e, but not RpL22e-like-PA, is SUMOylated and localizes to the nucleoplasm of Drosophila meiotic spermatocytes. Author : Kearse MG, Ireland JA, Prem SM, Chen AS, Ware VC.
    Nucleus. 2013 May-Jun;4(3):241-58. doi: 10.4161/nucl.25261. Epub 2013 Jun 6.
    PubMed® Id : 23778934
  • Glioma-specific cation conductance regulates migration and cell cycle progression. Author : Rooj AK, McNicholas CM, Bartoszewski R, Bebok Z, Benos DJ, Fuller CM.
    J Biol Chem. 2012 Feb 3;287(6):4053-65. doi: 10.1074/jbc.M111.311688. Epub 2011 Nov 30.
    PubMed® Id : 22130665
  • Interaction of ASIC1 and ENaC subunits in human glioma cells and rat astrocytes. Author : Kapoor N, Lee W, Clark E, Bartoszewski R, McNicholas CM, Latham CB, Bebok Z, Parpura V, Fuller CM, Palmer CA, Benos DJ.
    Am J Physiol Cell Physiol. 2011 Jun;300(6):C1246-59. doi: 10.1152/ajpcell.00199.2010. Epub 2011 Feb 23.
    PubMed® Id : 21346156
  • Proteolytic cleavage of human acid-sensing ion channel 1 by the serine protease matriptase. Author : Clark EB, Jovov B, Rooj AK, Fuller CM, Benos DJ.
    J Biol Chem. 2010 Aug 27;285(35):27130-43. doi: 10.1074/jbc.M110.153213. Epub 2010 Jul 2.
    PubMed® Id : 20601429
  • A Toxoplasma MORN1 null mutant undergoes repeated divisions but is defective in basal assembly, apicoplast division and cytokinesis. Author : Lorestani A, Sheiner L, Yang K, Robertson SD, Sahoo N, Brooks CF, Ferguson DJ, Striepen B, Gubbels MJ.
    PLoS One. 2010 Aug 19;5(8):e12302. doi: 10.1371/journal.pone.0012302.
    PubMed® Id : 20808817
  • The unique hypusine modification of eIF5A promotes islet beta cell inflammation and dysfunction in mice. Author : Maier B, Ogihara T, Trace AP, Tersey SA, Robbins RD, Chakrabarti SK, Nunemaker CS, Stull ND, Taylor CA, Thompson JE, Dondero RS, Lewis EC, Dinarello CA, Nadler JL, Mirmira RG.
    J Clin Invest. 2010 Jun;120(6):2156-70. doi: 10.1172/JCI38924. Epub 2010 May 24.
    PubMed® Id : 20501948
  • Knockdown of ASIC1 and epithelial sodium channel subunits inhibits glioblastoma whole cell current and cell migration. Author : Kapoor N, Bartoszewski R, Qadri YJ, Bebok Z, Bubien JK, Fuller CM, Benos DJ.
    J Biol Chem. 2009 Sep 4;284(36):24526-41. doi: 10.1074/jbc.M109.037390. Epub 2009 Jun 26.
    PubMed® Id : 19561078
  • Kv4 accessory protein DPPX (DPP6) is a critical regulator of membrane excitability in hippocampal CA1 pyramidal neurons. Author : Kim J, Nadal MS, Clemens AM, Baron M, Jung SC, Misumi Y, Rudy B, Hoffman DA.
    J Neurophysiol. 2008 Oct;100(4):1835-47. doi: 10.1152/jn.90261.2008. Epub 2008 Jul 30.
    PubMed® Id : 18667548
  • Localization of the phosphoethanolamine methyltransferase of the human malaria parasite Plasmodium falciparum to the Golgi apparatus. Author : Witola WH, Pessi G, El Bissati K, Reynolds JM, Mamoun CB.
    J Biol Chem. 2006 Jul 28;281(30):21305-11. Epub 2006 May 16.
    PubMed® Id : 16704982

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. Abgent's anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Abgent's anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.

REFERENCES

Ward, W. W., et al.(1980) Photochem. Photobiol. 31:611