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GST Tag Antibody

Purified Mouse Monoclonal Antibody (Mab)

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  • WB - GST Tag Antibody AM1011a
    Western blot analysis of lysate from 12 tag recombinant protein, using GST Tag Antibody(Cat. #AM1011a). AM1011a was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35μg.
  • WB - GST Tag Antibody AM1011a
    The anti-GST Mab (Cat. #AM1011a) is used in Western blot to detect GST recombinant protein purified from bacterial lysate.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 9AT106
Calculated MW 26000 Da
Additional Information
Other Names Glutathione S-transferase
Target/Specificity Purified recombinant GST fusion protein was used to produced this monoclonal antibody.
Dilution WB~~1:2,000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGST Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations ( 0 )


Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the Abgent anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.


Smith, D.B. and Johnson, K.S., (1988). Gene 67, 31.
Parker, M.W. et al., (1990) J. Mol. Biol. 213, 221.
Toye, B. et al., (1990) Infect. Immun. 58, 3909.
Guan, K.L. and Dixon, J.E. (1991) Anal. Biochem. 192, 262

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$ 295.00
$ 99.00
Cat# AM1011a
(40 western blots)
Availability: In Stock
Bulk Size
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