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>   home   >   Products   >   Primary Antibodies   >   AC133 (CD133) Antibody   

AC133 (CD133) Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • IHC-P - AC133 (CD133) Antibody AM1130a
    Localization of PROM1 protein in testicular tissues (A) Positive staining of PROM1 in the stereocilia (arrow) of epididymal duct in an adult. The inset presents a corresponding negative control. (B) Sporadic expression of PROM1 protein was visible in testis with regular spermatogenesis. The arrow points to a spermatogonia with a slightly stained cytoplasm. (C) PROM1 was present in membranous compartment of intratubular tumour cells as marked by the arrow. (D) Solid arrow marks the positive, membranous staining of seminoma cells for PROM1. Note the cytoplasmatic staining in several cells and the positive signal in stromal compartment including leukocytes. (E) Representative staining of PROM1 in testicular tissues from the 22nd week of gestation. The arrows mark the localization of PROM1 in gonocytes to a membrane and cytoplasmatic region. (F) No distinct PROM1 signal could be observed in third trimester of gestation (here, 30th week of gestation is presented)
  • WB - AC133 (CD133) Antibody AM1130a
    Western blot analysis of cell lysates from Y79 cell lines using AC133 antibody am1130a. Immunofluorescence analysis of Bone Marrow Mononuclear Cells stained with monoclonal CD133. Image courtesy of Rick Cohen from the Coriell Institute for Medical Research (NJ, USA).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession O43490
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 32AT1672
Calculated MW 97202 Da
Additional Information
Gene ID 8842
Other Names Prominin-1, Antigen AC133, Prominin-like protein 1, CD133, PROM1, PROML1
Target/Specificity This monoclonal antibody is generated from mice immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of AC133.
Dilution IHC-P~~1:10~50
WB~~1:100~500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAC133 (CD133) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PROM1
Synonyms PROML1
Function May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol- containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
Cellular Location Apical cell membrane; Multi- pass membrane protein. Cell projection, microvillus membrane; Multi-pass membrane protein Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Note=Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine
Tissue Location Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level).
Citations (0)

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Background

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.

References

Prognostic impact of the expression of putative cancer stem cell markers CD133, CD166, CD44s, EpCAM, and ALDH1 in colorectal cancer. Lugli A, et al. Br J Cancer, 2010 Jul 27. PMID 20606680. Members of the low-density lipoprotein receptor-related proteins provide a differential molecular signature between parental and CD133+ DAOY medulloblastoma cells. Annabi B, et al. Mol Carcinog, 2010 Jul. PMID 20564348. Activation of Akt and MAPK pathways enhances the tumorigenicity of CD133+ primary colon cancer cells. Wang YK, et al. Carcinogenesis, 2010 Aug. PMID 20530554. Demethylation of the CD133 gene is frequently detected in early gastric carcinoma. Hibi K, et al. Anticancer Res, 2010 Apr. PMID 20530428. The stem cell marker CD133 associates with enhanced colony formation and cell motility in colorectal cancer. Elsaba TM, et al. PLoS One, 2010 May 19. PMID 20502714.

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$ 295.00
$ 99.00
Cat# AM1130a
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(40 western blots)
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