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SET07 AntibodyPurified Mouse Monoclonal Antibody (Mab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AM1191a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
SET07 Antibody - Product info | |
| Application | WB, IHC, IF
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| Primary Accession | Q9NQR1 |
| Reactivity | Human, Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Mouse IgG1 |
| Clone Names | 43AT551.86 |
| Calculated MW | 42890 Da |
SET07 Antibody - Additional info | |
| Gene ID 387893 | |
| Other Names SETD8; KMT5A; PRSET7; SET07; SET8; N-lysine methyltransferase SETD8; H4-K20-HMTase SETD8; Histone-lysine N-methyltransferase SETD8; Lysine N-methyltransferase 5A; PR/SET domain-containing protein 07; SET domain-containing protein 8 | |
| Target/Specificity This SET07 antibody was raised using purified recombinant GST fusion protein encoding the N-terminal region of human SET07. | |
| Dilution WB~~1:100~500 IHC~~1:10~50 IF~~1:10~50 | |
| Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions SET07 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
SET07 Antibody - Protein Information | |
| Name SETD8 | |
| Synonyms KMT5A, PRSET7, SET07, SET8 | |
| Function Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes | |
| Cellular Location Nucleus. Chromosome. Note=Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin | |
SET07 Antibody - Related products
AP1191a: SET07 Antibody (N-term)
AP1191b: SET07 Antibody (C-term)
AP6557c: SETD8 Antibody (Center)
RI14797: SETD8 predesign siRNA
DC07781: Human SETD8 cDNA Clone
LY11736a: SETD8 Over-expression Lysate
BP1191a: SET07 Antibody (N-term) Blocking Peptide
SET07 Antibody - Application data
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The anti-SET07 Mab (Cat. #AM1191a) is used in Western blot to detect SET07 in SAOS-2 (lane 1) and NIH/3T3 cell lysates (lane 2).
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Formalin-fixed and paraffin-embedded human testis tissue reacted with SET07 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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(TOP)Negative control of hela cells without PE-conjugated goat anti-mouse lgG (whole molecule).PE-conjugated goat anti-mouse lgG emits red fluorescence. ( Bottom)Immunofluorescence analysis of SET07 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule).
SET07 Antibody - Research Areas
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REFERENCES
An miR-502-binding site single-nucleotide polymorphism in the 3'-untranslated region of the SET8 gene is associated with early age of breast cancer onset. Song F, et al. Clin Cancer Res, 2009 Oct 1. PMID 19789321. Product specificity and mechanism of protein lysine methyltransferases: insights from the histone lysine methyltransferase SET8. Zhang X, et al. Biochemistry, 2008 Jun 24. PMID 18512960. Catalytic function of the PR-Set7 histone H4 lysine 20 monomethyltransferase is essential for mitotic entry and genomic stability. Houston SI, et al. J Biol Chem, 2008 Jul 11. PMID 18480059. PR-Set7 establishes a repressive trans-tail histone code that regulates differentiation. Sims JK, et al. Mol Cell Biol, 2008 Jul. PMID 18474616. SET8 plays a role in controlling G1/S transition by blocking lysine acetylation in histone through binding to H4 N-terminal tail. Yin Y, et al. Cell Cycle, 2008 May 15. PMID 18418072.
