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UBC9 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • WB - UBC9 Antibody AM1261a
    All lanes : Anti-UBC9 Antibody at 1:1000-1:2000 dilution Lane 1: human heart lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 18 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - UBC9 Antibody AM1261a
    Western blot analysis of anti-UBC9 Antibody (Cat. #AM1261a) in Ramos cell line lysates (35μg/lane). UBC9(arrow) was detected using the purified Mab.
    detail
  • SPECIFICATION
  • CITATIONS: 5
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P63279
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone/Animal Names 67AT1273.95.90
Calculated MW 18007 Da
Additional Information
Gene ID 7329
Other Names SUMO-conjugating enzyme UBC9, 632-, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18, UBE2I, UBC9, UBCE9
Target/Specificity This UBC9 antibody was raised using purified GST-UBC9 fusion protein.
Dilution WB~~1:500~1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBC9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBE2I
Synonyms UBC9, UBCE9
Function Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity (PubMed:26620705).
Cellular Location Nucleus. Cytoplasm Cytoplasm, perinuclear region Note=Mainly nuclear (By similarity). In spermatocytes, localizes in synaptonemal complexes (PubMed:8610150). Recruited by BCL11A into the nuclear body (By similarity). {ECO:0000250|UniProtKB:P63280, ECO:0000269|PubMed:8610150}
Tissue Location Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Research Areas
Citations ( 0 )

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

References

Expression analysis of Ubc9, the single small ubiquitin-like modifier (SUMO) E2 conjugating enzyme, in normal and malignant tissues. Moschos SJ, et al. Hum Pathol, 2010 Sep. PMID 20561671. Ubc9 promotes breast cell invasion and metastasis in a sumoylation-independent manner. Zhu S, et al. Oncogene, 2010 Mar 25. PMID 20023705. Association of SUMO1 and UBC9 genotypes with tumor response in non-small-cell lung cancer treated with irinotecan-based chemotherapy. Han JY, et al. Pharmacogenomics J, 2010 Apr. PMID 19859084. Characterization of papillomavirus E1 helicase mutants defective for interaction with the SUMO-conjugating enzyme Ubc9. Fradet-Turcotte A, et al. Virology, 2009 Dec 20. PMID 19836047. Ubc9 gene polymorphisms and late-onset Alzheimer's disease in the Korean population: a genetic association study. Ahn K, et al. Neurosci Lett, 2009 Nov 20. PMID 19765634.

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$ 365.00
$ 140.00
Cat# AM1261a
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