- CITATIONS: 3
|Application ||WB, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||18007 Da|
|Other Names||SUMO-conjugating enzyme UBC9, 632-, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18, UBE2I, UBC9, UBCE9|
|Target/Specificity||This UBC9 antibody was raised using purified GST-UBC9 fusion protein.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBC9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'.|
|Cellular Location||Nucleus. Cytoplasm. Note=Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body (By similarity).|
|Tissue Location||Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.|
Provided below are standard protocols that you may find useful for product applications.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
Expression analysis of Ubc9, the single small ubiquitin-like modifier (SUMO) E2 conjugating enzyme, in normal and malignant tissues. Moschos SJ, et al. Hum Pathol, 2010 Sep. PMID 20561671. Ubc9 promotes breast cell invasion and metastasis in a sumoylation-independent manner. Zhu S, et al. Oncogene, 2010 Mar 25. PMID 20023705. Association of SUMO1 and UBC9 genotypes with tumor response in non-small-cell lung cancer treated with irinotecan-based chemotherapy. Han JY, et al. Pharmacogenomics J, 2010 Apr. PMID 19859084. Characterization of papillomavirus E1 helicase mutants defective for interaction with the SUMO-conjugating enzyme Ubc9. Fradet-Turcotte A, et al. Virology, 2009 Dec 20. PMID 19836047. Ubc9 gene polymorphisms and late-onset Alzheimer's disease in the Korean population: a genetic association study. Ahn K, et al. Neurosci Lett, 2009 Nov 20. PMID 19765634.
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