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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NANOG Antibody   

NANOG Antibody

Mouse Monoclonal Antibody (Mab)

     
  • WB - NANOG Antibody AM1486b
    NANOG (Cat. #AM1486b) western blot analysis in K562 cell line lysates (35μg/lane).This demonstrates the NANOG antibody detected the NANOG protein (arrow).
  • IHC-P - NANOG Antibody AM1486b
    NANOG Antibody (Cat. #AM1486b)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjμgation of the secondary antibody and DAB staining.This data demonstrates the use of NANOG Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
  • WB - NANOG Antibody AM1486b
    Western blot analysis of anti-NANOG monoclonal antibody (Cat.#AM1486b) by NANOG recombinant protein. NANOG (NANOG + His tag)(arrow) was detected using the Mab.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9H9S0
Other Accession NP_079141.2, XP_002344676.1
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1,k
Clone Names 60CT77.1.1
Calculated MW 34620 Da
Additional Information
Gene ID 79923
Other Names Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG
Target/Specificity This NANOG monoclonal antibody is generated from mouse immunized with NANOG recombinant protein.
Dilution WB~~1:100~250
IHC-P~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNANOG Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NANOG
Function Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'- [CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108, ECO:0000269|PubMed:15983365}
Tissue Location Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
Research Areas
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Background

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).

References

Trubiani, O., et al. J. Cell. Physiol. 225(1):123-131(2010)
Po, A., et al. EMBO J. 29(15):2646-2658(2010)
Zbinden, M., et al. EMBO J. 29(15):2659-2674(2010)
Moretto-Zita, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(30):13312-13317(2010)
Kuijk, E.W., et al. PLoS ONE 5 (6), E10987 (2010) :

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$ 295.00
$ 99.00
Cat# AM1486b
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(40 western blots)
Availability: In Stock
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