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ATG16L1 Antibody (Ascites)Mouse Monoclonal Antibody (Mab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AM1817a | 0.1 ml 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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ATG16L1 Antibody (Ascites) - Product info | |
| Application | IF, WB
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| Primary Accession | Q676U5 |
| Reactivity | Human |
| Concentration | Crude ascites |
| Isotype | IgG1κ |
| Clone Names | 54CT27.2.6 |
| Calculated MW | 68265 Da |
ATG16L1 Antibody (Ascites) - Additional info | |
| Gene ID 55054 | |
| Other Names ATG16L1; APG16L; Autophagy-related protein 16-1; APG16-like 1 | |
| Target/Specificity Purified His-tagged ATG16L1 protein(Fragment) was used to produced this monoclonal antibody. | |
| Dilution IF~~1:200 WB~~1:2000 | |
| Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ATG16L1 Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ATG16L1 Antibody (Ascites) - Protein Information | |
| Name ATG16L1 | |
| Synonyms APG16L | |
| Function Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II | |
| Cellular Location Cytoplasm (By similarity). Preautophagosomal structure membrane; Peripheral membrane protein (By similarity) Note=Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5 (By similarity) | |
ATG16L1 Antibody (Ascites) - Related products
AM1817a: ATG16L1 Antibody (Ascites)
AP1817a: ATG16L Antibody (N-term)
AP1817c: ATG16L Antibody (C-term)
AP3366a: Phospho-ATG16L-S287 Antibody
AP3442a: Phospho-ATG16L-S213 Antibody
RI10452: ATG16L1 predesign siRNA
BP1817b: APG16L Antibody (L176) Blocking Peptide
BP1817c: APG16L Antibody (C-term) Blocking Peptide
BP1817d: APG16 Antibody Blocking Peptide
ATG16L1 Antibody (Ascites) - Application data
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Fluorescent image of U251 cells stained with AM1817a ATG16L1 antibody.U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1817a ATG16L1 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG16L1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000085978).
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Western blot analysis of anti-ATG16L1 Monoclonal Antibody (Cat.#AM1817a) by ATG16L1 recombinant protein (Fragment). ATG16L1 (Fragment) protein (arrow) was detected using the ascites Mab. (1:2000)
ATG16L1 Antibody (Ascites) - Research Areas
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BACKGROUND
The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.
REFERENCES
References for protein: 1.Age at onset in Huntington's disease is modified by the autophagy pathway: implication of the V471A polymorphism in Atg7. Metzger S, et al. Hum Genet, 2010 Oct. PMID 20697744. 2.Plasma membrane contributes to the formation of pre-autophagosomal structures. Ravikumar B, et al. Nat Cell Biol, 2010 Aug. PMID 20639872. 3.Replication and meta-analysis of 13,000 cases defines the risk for interleukin-23 receptor and autophagy-related 16-like 1 variants in Crohn's disease. Cotterill L, et al. Can J Gastroenterol, 2010 May. PMID 20485703. 4.Is there a role for Crohn's disease-associated autophagy genes ATG16L1 and IRGM in formation of granulomas? Wolfkamp SC, et al. Eur J Gastroenterol Hepatol, 2010 Aug. PMID 20395867. 5.NOD2/CARD15, ATG16L1 and IL23R gene polymorphisms and childhood-onset of Crohn's disease. Gazouli M, et al. World J Gastroenterol, 2010 Apr 14. PMID 20380008. References for U251 cell line: 1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449]. 2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950]. 3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].
