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NUCL Antibody

Purified Mouse Monoclonal Antibody (Mab)

  • WB - NUCL Antibody AM1824a
    Western blot analysis of NUCL Antibody (Cat. #AM1824a) in 293 cell line lysates (35μg/lane). NUCL (arrow) was detected using the purified Mab.
  • IHC-P - NUCL Antibody AM1824a
    NUCL Monoclonal Antibody (Cat. #AM1824A) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjμgation of the secondary antibody and DAB staining.This data demonstrates the use of NUCL Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - NUCL Antibody AM1824a
    Confocal immunofluorescent analysis of NUCL Antibody(Cat#AM1824a) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P19338
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1?
Clone Names 130CT43.8.5
Calculated MW 76614 Da
Additional Information
Gene ID 4691
Other Names Nucleolin, Protein C23, NCL
Target/Specificity This NUCL Monoclonal antibody is generated from mouses immunized with a protein from human NUCL.
Dilution WB~~1:500~1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNUCL Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCL
Function Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Cellular Location Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs
Citations (0)

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Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.


Ishimaru, D., et al. J. Biol. Chem. 285(35):27182-27191(2010) Tulchin, N., et al. Am. J. Pathol. 176(3):1203-1214(2010) Strang, B.L., et al. J. Virol. 84(4):1771-1784(2010) Bertrand, L., et al. J. Virol. 84(1):109-118(2010) Jerke, U., et al. PLoS ONE 4 (12), E8302 (2009)

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$ 295.00
$ 99.00
Cat# AM1824a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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