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DLG4 Antibody (C-term) (Rat) (Ascites)
Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P31016 |
| Other Accession | Q62108, P78352 |
| Reactivity | Human, Mouse |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgM |
| Clone/Animal Names | 127CT15.8.20 |
| Calculated MW | 80465 Da |
| Antigen Region | 677-706 aa |
| Gene ID | 29495 |
|---|---|
| Other Names | Disks large homolog 4, Postsynaptic density protein 95, PSD-95, Synapse-associated protein 90, SAP-90, SAP90, Dlg4, Dlgh4, Psd95 |
| Target/Specificity | This DLG4 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 677-706 amino acids from the C-terminal region of human DLG4. |
| Dilution | WB~~1:500~1000 IHC-P~~1:50~100 |
| Format | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DLG4 Antibody (C-term) (Rat) (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Dlg4 {ECO:0000312|RGD:68424} |
|---|---|
| Function | Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins (PubMed:15317815, PubMed:15358863, PubMed:19596852, PubMed:23300088, PubMed:26679993, PubMed:20628354). Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B (By similarity). Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression (PubMed:19596852).Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins (By similarity). |
| Cellular Location | Cell membrane; Lipid-anchor; Cytoplasmic side. Postsynaptic density. Synapse. Cytoplasm Cell projection, axon. Cell projection, dendritic spine. Cell projection, dendrite. Presynapse Note=High levels in postsynaptic density of neurons in the forebrain Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. Suppression of neuronal activity induces synaptic accumulation and clustering of DLG4 (PubMed:19596852). |
| Tissue Location | Expressed in brain (at protein level) (PubMed:12151521, PubMed:27307232, PubMed:20962234). Detected in juxtaparanodal zones in the central nervous system and at nerve terminal plexuses of basket cells in the cerebellum (PubMed:20089912) Expressed in cerebrum (PubMed:27307232). Expressed in hippocampal neurons (at protein level) (PubMed:11502259, PubMed:12151521, PubMed:27307232, PubMed:27756895). Isoform 1 and isoform 2: highly expressed in cerebellum, cortex, hippocampus, and corpus striatum (PubMed:12151521, PubMed:20962234). |
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Provided below are standard protocols that you may find useful for product applications.
Background
a molecular scaffolding protein that binds and clusters N-methyl-D-aspartate receptors at neuronal synapses; may be involved in guanine nucleotide-mediated signal transduction pathway [RGD]
References
SAP102 is a highly mobile MAGUK in spines. Zheng CY, et al. J Neurosci, 2010 Mar 31. PMID 20357126. Synaptic clustering of PSD-95 is regulated by c-Abl through tyrosine phosphorylation. de Arce KP, et al. J Neurosci, 2010 Mar 10. PMID 20220006. Transduced PDZ1 domain of PSD-95 decreases Src phosphorylation and increases nNOS (Ser847) phosphorylation contributing to neuroprotection after cerebral ischemia. Wang WW, et al. Brain Res, 2010 Apr 30. PMID 20197063. Hidden dynamic allostery in a PDZ domain. Petit CM, et al. Proc Natl Acad Sci U S A, 2009 Oct 27. PMID 19828436. Integrin-linked kinase is involved in cocaine sensitization by regulating PSD-95 and synapsin I expression and GluR1 Ser845 phosphorylation. Chen Q, et al. J Mol Neurosci, 2010 Mar. PMID 19629758.
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