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Rat Serpinh1 Antibody (Center)

Purified Mouse Monoclonal Antibody (Mab)

     
  • WB - Rat Serpinh1 Antibody (Center) AM1834a
    Western blot analysis of RAT SERPH Antibody (Center) (Cat. #AM1834a) in rat heart tissue lysates (35μg/lane).SERPH (arrow) was detected using the purified Mab.(1:100)
    detail
  • IHC-P - Rat Serpinh1 Antibody (Center) AM1834a
    RAT SERPH Monoclonal Antibody (Center) (Cat. #AM1834a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjμgation of the secondary antibody and DAB staining. This data demonstrates the use of the RAT SERPH Monoclonal Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P29457
Other Accession P19324, P50454, Q2KJH6
Reactivity Human, Rat
Predicted Bovine, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone/Animal Names 134CT3.1.1
Calculated MW 46518 Da
Antigen Region 222-251 aa
Additional Information
Gene ID 29345
Other Names Serpin H1, 47 kDa heat shock protein, Collagen-binding protein, Colligin, GP46, Serpinh1, Cbp1, Hsp47
Target/Specificity This Rat Serpinh1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 222-251 amino acids from the Central region of rat Serpinh1.
Dilution WB~~1:500~1000
IHC-P~~1:50~100
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRat Serpinh1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Serpinh1
Synonyms Cbp1, Hsp47
Function Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Cellular Location Endoplasmic reticulum lumen.
Citations (0)
citation

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References

Localization of HSP47 mRNA in murine bleomycin-induced pulmonary fibrosis. Kakugawa T, et al. Virchows Arch, 2010 Mar. PMID 20094730. The level of the transcription factor Pax6 is essential for controlling the balance between neural stem cell self-renewal and neurogenesis. Sansom SN, et al. PLoS Genet, 2009 Jun. PMID 19521500. Fog2 is critical for cardiac function and maintenance of coronary vasculature in the adult mouse heart. Zhou B, et al. J Clin Invest, 2009 Jun. PMID 19411759. Adenovirus-mediated RNA interference against collagen-specific molecular chaperone 47-KDa heat shock protein suppresses scar formation on mouse wounds. Wang Z, et al. Cell Biol Int, 2008 May. PMID 18093850. Type I collagen in Hsp47-null cells is aggregated in endoplasmic reticulum and deficient in N-propeptide processing and fibrillogenesis. Ishida Y, et al. Mol Biol Cell, 2006 May. PMID 16525016.

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$ 365.00
$ 140.00
Cat# AM1834a
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