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AFP Antibody (N-term)

Mouse Monoclonal Antibody (Mab)

  • WB - AFP Antibody (N-term) AM1835a
    Anti- at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - AFP Antibody (N-term) AM1835a
    "Anti-AFP Antibody (N-term) at 1:31.25 dilution + MCF-7 whole cell lysate Secondary Goat Anti-mouse IgM, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68678 Da Blocking/Dilution buffer: 5% NFDM/TBST."
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P02771
Other Accession NP_001125.1
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse IgM
Clone Names 143CT50.11.8
Calculated MW 68678 Da
Antigen Region 56-85 aa
Additional Information
Gene ID 174
Other Names Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP
Target/Specificity This AFP antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from the N-terminal region of human AFP.
Dilution WB~~1:31.25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAFP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AFP
Synonyms HPAFP
Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Cellular Location Secreted.
Tissue Location Plasma. Synthesized by the fetal liver and yolk sac
Research Areas
Citations (0)

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alpha 1 Fetoprotein encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.


Suriapranata, I.M., et al. Clin. Chim. Acta 411 (5-6), 351-358 (2010)
Vibert, E., et al. Am. J. Transplant. 10(1):129-137(2010)
Potapovich, A.I., et al. Br. J. Pharmacol. 158(5):1236-1247(2009)

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$ 295.00
$ 99.00
Cat# AM1835a
(40 western blots)
Availability: In Stock
Bulk Size
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