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>   home   >   Products   >   Primary Antibodies   >   Rat Npm1 Antibody (Ascites)   

Rat Npm1 Antibody (Ascites)

Purified Mouse Monoclonal Antibody (Mab)

     
  • WB - Rat Npm1 Antibody (Ascites) AM1837a
    Western blot analysis of RAT Npm1 Antibody (Cat. #AM1837a) in rat heart tissue lysates (35μg/lane).NPM (arrow) was detected using the purified Mab.(1:2000)
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P13084
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1,Igκ
Clone Names 3A9F1
Calculated MW 32560 Da
Additional Information
Gene ID 25498
Other Names Nucleophosmin, NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, Npm1
Target/Specificity This RAT NPM antibody is generated from mouse immunized with RAT NPM recombinant protein.
Dilution WB~~1:500~2000
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRat Npm1 Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Npm1
Function Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules (By similarity). Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication (By similarity). Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation (By similarity).
Cellular Location Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Colocalizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. NEK2 is required for its localization to the centrosome during mitosis (By similarity).
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Background

RNA-associated nucleolar phosphoprotein; involved in ribosome assembly [RGD]

References

Protein B23/nucleophosmin/numatrin nuclear dynamics in relation to protein kinase CK2 and apoptotic activity in prostate cells. Wang G, et al. Biochemistry, 2010 May 11. PMID 20387789. Nucleophosmin may act as an alarmin: implications for severe sepsis. Nawa Y, et al. J Leukoc Biol, 2009 Sep. PMID 19581374. Disruption of ATP binding destabilizes NPM/B23 and inhibits anti-apoptotic function. Choi JW, et al. BMB Rep, 2008 Dec 31. PMID 19123973. Nuclear Akt interacts with B23/NPM and protects it from proteolytic cleavage, enhancing cell survival. Lee SB, et al. Proc Natl Acad Sci U S A, 2008 Oct 28. PMID 18931307. Ebp1 association with nucleophosmin/B23 is essential for regulating cell proliferation and suppressing apoptosis. Okada M, et al. J Biol Chem, 2007 Dec 14. PMID 17951246.

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$ 295.00
Cat# AM1837a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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