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MAP2 Antibody (Ascites)

Mouse Monoclonal Antibody (Mab)

     
  • IF - MAP2 Antibody (Ascites) AM1853a
    Confocal immunofluorescent analysis of MAP2 Antibody (Cat#AM1853a) with brain tissue followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • IHC - MAP2 Antibody (Ascites) AM1853a
    Immunohistochemical analysis of paraffin-embedded Human brain section using Pink1(Cat#AM1853a). AM1853a was diluted at 1:500 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
    detail
  • WB - MAP2 Antibody (Ascites) AM1853a
    MAP2 Antibody (Cat. #AM1853a) western blot analysis in MCF-7 cell line lysates (15μg/lane).This demonstrates the MAP2 antibody detected the MAP2 protein (arrow).(1:1000)
    detail
  • IHC-P - MAP2 Antibody (Ascites) AM1853a
    MAP2 Antibody (Ascites) (Cat. #AM1853a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjμgation of the secondary antibody and DAB staining.This data demonstrates the use of MAP2 Antibody (Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
  • CITATIONS: 1
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IF, WB, E
Primary Accession P11137
Other Accession NP_002365.3
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1,K
Clone/Animal Names 159CT34.12.3.4
Calculated MW 199526 Da
Additional Information
Gene ID 4133
Other Names Microtubule-associated protein 2, MAP-2, MAP2
Target/Specificity This MAP2 Monoclonal antibody was raised using purified His-tagged recombinant human MAP2.
Dilution IF~~1:10~50
IHC~~1:500
WB~~1:1000
IHC-P~~1:10~50
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAP2 Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP2
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Cellular Location Cytoplasm, cytoskeleton. Cell projection, dendrite {ECO:0000250|UniProtKB:P20357}
Research Areas
Citations ( 0 )

Background

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

References

Albala J.S.et.al. Gene 136:377-378(1993).

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$ 182.50
Cat# AM1853a
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