|Application ||WB, IF, E|
|Calculated MW||36092 Da|
|Other Names||ELAV-like protein 1, Hu-antigen R, HuR, ELAVL1, HUR|
|Target/Specificity||This ELAVL1 monoclonal antibody is generated from mouse immunized with ELAVL1 recombinant protein.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ELAVL1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed:14517288, PubMed:18285462). Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation (By similarity). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:8626503, PubMed:17632515, PubMed:18285462, PubMed:23519412). Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'- UUUU[AG]UUU-3' motif in vitro (PubMed:8626503). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity).|
|Cellular Location||Cytoplasm. Nucleus. Note=Translocates into the cytoplasm following phosphorylation by MAPKAPK2 (PubMed:14517288). Likewise, phosphorylation by PRKCD promotes translocation from the nucleus into the cytoplasm, where it is associated with free and cytoskeleton-bound polysomes (PubMed:18285462)|
|Tissue Location||Ubiquitous. Detected in brain, liver, thymus and muscle.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It destabilizes mRNAs and thereby regulates gene expression.
Tholanikunnel, B.G., et al. J. Biol. Chem. 285(44):33816-33825(2010)
Drury, G.L., et al. J. Biol. Chem. 285(41):31130-31138(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Raspaglio, G., et al. Cancer Res. 70(14):5891-5900(2010)
Ahn, J., et al. Retrovirology 7, 40 (2010) :
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