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MASTL Antibody

Mouse Monoclonal Antibody (Mab)

     
  • IF - MASTL Antibody AM1911B
    Confocal immunofluorescent analysis of MASTL Antibody (Cat#AM1911b) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - MASTL Antibody AM1911B
    MASTL (Cat. #AM1911b) western blot analysis in HepG2 cell line lysates (35μg/lane).This demonstrates the MASTL antibody detected the MASTL protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q96GX5
Other Accession NP_001165774.1
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1,k
Clone/Animal Names 235CT7.8.2.3
Calculated MW 97319 Da
Additional Information
Gene ID 84930
Other Names Serine/threonine-protein kinase greatwall, GW, GWL, hGWL, Microtubule-associated serine/threonine-protein kinase-like, MAST-L, MASTL, GW, GWL, THC2
Target/Specificity This MASTL monoclonal antibody is generated from mouse immunized with MASTL recombinant protein.
Dilution IF~~1:10~50
WB~~1:500~1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMASTL Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MASTL
Synonyms GW, GWL, THC2
Function Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser- 62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Cleavage furrow. Note=During interphase is mainly nuclear, upon nuclear envelope breakdown localizes at the cytoplasm and during mitosis at the centrosomes. Upon mitotic exit moves to the cleavage furrow.
Research Areas
Citations (0)
citation

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Background

This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus.

References

Gandhi, M.J., et al. Hum. Hered. 55(1):66-70(2003)
Drachman, J.G., et al. Blood 96(1):118-125(2000)
Savoia, A., et al. Am. J. Hum. Genet. 65(5):1401-1405(1999)

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$ 365.00
$ 140.00
Cat# AM1911B
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