|Application ||WB, IF, E|
|Calculated MW||97319 Da|
|Other Names||Serine/threonine-protein kinase greatwall, GW, GWL, hGWL, Microtubule-associated serine/threonine-protein kinase-like, MAST-L, MASTL, GW, GWL, THC2|
|Target/Specificity||This MASTL monoclonal antibody is generated from mouse immunized with MASTL recombinant protein.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MASTL Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||GW, GWL, THC2|
|Function||Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.|
|Cellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Cleavage furrow Note=During interphase is mainly nuclear, upon nuclear envelope breakdown localizes at the cytoplasm and during mitosis at the centrosomes. Upon mitotic exit moves to the cleavage furrow|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus.
Gandhi, M.J., et al. Hum. Hered. 55(1):66-70(2003)
Drachman, J.G., et al. Blood 96(1):118-125(2000)
Savoia, A., et al. Am. J. Hum. Genet. 65(5):1401-1405(1999)
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