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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   POU5F1 Antibody (Ascites)   

POU5F1 Antibody (Ascites)

Mouse Monoclonal Antibody (Mab)

  • WB - POU5F1 Antibody (Ascites) AM1967A
    POU5F1 Antibody (Cat. #AM1967a) western blot analysis in K562 cell line lysates (35μg/lane).This demonstrates the POU5F1 antibody detected the POU5F1 protein (arrow).
  • WB - POU5F1 Antibody (Ascites) AM1967A
    POU5F1 Antibody (Cat. #AM1967a) western blot analysis in mouse brain tissue lysates (35μg/lane).This demonstrates the POU5F1 antibody detected the POU5F1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q01860
Other Accession NP_002692.2
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgM
Clone Names 341CT7.6.2
Calculated MW 38571 Da
Additional Information
Gene ID 5460
Other Names POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, POU5F1, OCT3, OCT4, OTF3
Target/Specificity This POU5F1 monoclonal antibody is generated from mouse immunized with POU5F1 recombinant protein.
Dilution WB~~1:500~4000
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOU5F1 Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POU5F1
Synonyms OCT3, OCT4, OTF3
Function Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Cellular Location Cytoplasm. Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern
Tissue Location Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Research Areas
Citations (0)

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This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.


Abu-Remaileh, M., et al. EMBO J. 29(19):3236-3248(2010)
Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
Schultz, S.S., et al. Mol. Cell. Biol. 30(18):4521-4534(2010)
Kim, S., et al. Br. J. Cancer 102(2):436-446(2010)
Wang, X., et al. Stem Cells 27(6):1265-1275(2009)

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$ 295.00
Cat# AM1967A
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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