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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Bmi1 Antibody   

Mouse Bmi1 Antibody

Mouse Monoclonal Antibody (Mab)

     
  • WB - Mouse Bmi1 Antibody AM1980b
    Mouse Bmi1 antibody (Cat. #AM1980b) western blot analysis in mouse cerebellum tissue lysates (35μg/lane).This demonstrates the Mouse Bmi1 antibody detected the Mouse Bmi1 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P25916
Other Accession NP_031578.2
Reactivity Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names 353CT22.4.4
Calculated MW 36708 Da
Antigen Region 136-163 aa
Additional Information
Gene ID 12151
Other Names Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, Bmi1, Bmi-1, Pcgf4
Target/Specificity This Mouse Bmi1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 136-163 amino acids from mouse Bmi1.
Dilution WB~~1:200~1600
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Bmi1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Bmi1
Synonyms Bmi-1, Pcgf4
Function Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2 (By similarity).
Cellular Location Nucleus. Cytoplasm.
Tissue Location Detected in most organs with high expression levels in thymus, heart, brain and testis
Research Areas
Citations (0)

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Background

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (By similarity). In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

References

Pereira, J.D., et al. Proc. Natl. Acad. Sci. U.S.A. 107(36):15957-15962(2010)
Chiba, T., et al. Hepatology 52(3):1111-1123(2010)
Maertens, G.N., et al. EMBO J. 29(15):2553-2565(2010)
Lee, K.S., et al. Oncogene 29(23):3349-3361(2010)
Schaller, M.A., et al. PLoS ONE 5 (8), E12172 (2010) :

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Discontinued
Cat# AM1980b
Size:
(40 western blots)
Alternative Products:

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