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LGR5/GPR49 Antibody (Ascites)

Mouse Monoclonal Antibody (Mab)

  • WB - LGR5/GPR49 Antibody (Ascites) AM1992a
    LGR5/GPR49 Antibody (Cat. #AM1992a) western blot analysis in K562 cell line lysates (35μg/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75473
Other Accession F1MT22, NP_003658.1
Reactivity Human
Predicted Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 349CT16.1.3.1
Calculated MW 99998 Da
Antigen Region 443-473 aa
Additional Information
Gene ID 8549
Other Names Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, LGR5, GPR49, GPR67
Target/Specificity This LGR5/GPR49 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 443-473 amino acids from human LGR5/GPR49.
Dilution WB~~1:1000~8000
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLGR5/GPR49 Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LGR5
Synonyms GPR49, GPR67
Function Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R- spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Cellular Location Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Note=Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans- Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven (PubMed:23439653).
Tissue Location Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver
Research Areas
Citations (0)

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Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.


Fontaine-Bisson, B., et al. Diabetologia 53(10):2155-2162(2010)
Voight, B.F., et al. Nat. Genet. 42(7):579-589(2010)
Uchida, H., et al. Cancer Sci. 101(7):1731-1737(2010)
Fan, X.S., et al. Int J Colorectal Dis 25(5):583-590(2010)
Acevedo, A.C., et al. J. Dent. Res. 89(2):128-132(2010)

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$ 295.00
Cat# AM1992a
(40 western blots)
Availability: In Stock
Bulk Size
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